Saturday, May 4, 2024

The Ultimate Email Design Guide & Best Practices 2021

mail design

Always take a step back and ask yourself how you would react to this message if you were a customer seeing this email for the first time. Most people are familiar with the concept of hiring a freelance designer. But it isn’t the only way to start working with an email template designer. If you want a lot of different takes on an email template, using your company’s colors and logo, a design contest is the way to go. Not only does responsive email design matter for accessibility, but it's non-negotiable to create a better experience for your readers.

Stay brand-friendly

(You’ll know how to do the same by the time you’re done with this post). Create an amazing static website in minutes and export ready-to-use template. Get started with the most advanced email builder for your next campaign or newsletter. Nataly is an exceptional web designer and developer with a master's degree in computer science. Nataly remains at the forefront of the industry by staying updated with the latest trends through continuous learning.

The InfoQ Newsletter

However, there should only be a single primary CTA button, while the other secondary ones must support it. As an email marketer, Call To Action or CTA buttons should be your favorite email design element since they directly impact conversions. But including generic ‘Download’ buttons or worse, only hyperlinking text won’t do you any good. The bright blue color is carried through to the corresponding website, making this a strong example of seamless branding. Thankfully, there are plenty of email marketing geeks out there (ourselves included) that do think all of that's kind of fun. These less glamorous aspects of email marketing — though critical to your campaign's success — don't paint the entire picture of what amazing email marketing really is.

Craft a strong subject line.

If you want to delve into the nuances of email design, look no further. We have compiled a list of the key features of email design and how you can optimize those to create captivating email design campaigns. When it comes to email marketing, email design is often the unsung hero. They provide quick information when you want instant answers. The following FAQs relating to email design have been answered, so you’re always on the top of your game. The harmonious color palette is the focal point of this email template.

Optimize emails for dark mode

He founded Designmodo, a reputable company specializing in website and email building. Andrian shares his knowledge through guest lectures, interviews, and online publications, and is a respected voice in the industry. Much like the previous email design, this one also takes advantage of one of the biggest trends, animated gif. However, unlike Adobe’s newsletter, where it plays a supporting role, here it runs the show.

Target audience

We are living in a world with a tremendous amount of information. You can’t expect your audience, no matter how engaged with your brand, to read everything. A great way to streamline the process of working on and incorporate all 13 of the above best practices is to use email design tools and software. Calls-to-action (CTAs) are used to convert your email recipients. For instance, you can use a CTA to get recipients to follow you on social media, visit your website, chat with a sales rep, or become paying customers.

These examples showcase innovative layouts, captivating visuals, and strategic use of color and typography. By studying these examples, you can gain insights into what works best in terms of engaging your audience and driving conversions when you design an email. The right email layout design for your newsletter depends on many factors, one of which being content, which we’ll cover in the next section.

Visuals

In the end, there is an eye-catching call-to-action button that directs readers exactly to the selling page. With Startup App and Slides App you can build unlimited websites using the online website editor which includes ready-made designed and coded elements, templates and themes. Since email marketing is not going anywhere soon, and great expectations lie in store for it, it is vital to understand the basics. Let’s dive into email design, consider essential elements, find out how to build a modern high-converting digital newsletter, and get inspiration from real-life examples. We're firm believers in the Golden Rule, which is why editorial opinions are ours alone and have not been previously reviewed, approved, or endorsed by included advertisers. The Ascent, a Motley Fool service, does not cover all offers on the market.

GET INSPIRED: EMAIL NEWSLETTER LOOKBOOK

Besides, color themes should change depending on the type of campaign you're creating. For instance, a holiday email campaign should have a color that signifies that occasion. A Christmas email should use a red, blue, and green color theme, while a black Friday email should have a black or red theme.

This simple, single-column email layout design is perfect for creating a newsletter. They are great to differentiate multiple marketing messages and keep the text easy to read. Sticking to a comfortable pace, it promotes the diversity and the beauty of the new collection. Although it is not recommended to rely solely on visuals nevertheless, with alt text, you can make it happen. Mystery email design is one of the most powerful marketing tricks. Can you resist the temptation of hitting this button and find out what waits for you?

The coloring and graphics stand behind a beautiful festive aesthetics. The team plays with a vibrant pattern and overly decorative font. The pattern is used as a background, whereas typeface is set to a large size and used only for headlines. The offer has a solid foundation thanks to a generous amount of white space and a relatively big size.

Download Visme's iOS app to view and make edits to your email designs on the go. The mobile-friendly interface allows you to see how your email will appear on various screens, including iPhone and iPad. This layout is characterized by a top header that includes the headline or main message, followed by a subheading and then a column of text on the left side.

mail design

Generally, it’s advised to make preheader text no more than about 130 characters. And if you’re not satisfied with how it looks, ou can always change the colors and fonts of the email to align with your branding guidelines, using My Brand Kit. Email newsletters about new services can also help you explain them in more depth. While the previous example is simple and merely informs the user of a design update, in this example, the sender provides specific details about a new service and how to access it. Similarly, offering team members actionable advice is another great reason to consider a simple email design approach, such as this one.

Designer TOM RAFFIELD found fame when his hand-built house was featured on Grand Designs. Now he tells Samuel - Daily Mail

Designer TOM RAFFIELD found fame when his hand-built house was featured on Grand Designs. Now he tells Samuel.

Posted: Sat, 16 Mar 2024 07:00:00 GMT [source]

A/B testing (AKA “split testing”) involves sending two versions of an email at the same time to see which design works best. It takes all the guesswork out of email design, allowing you to make tweaks based on hard data. And thanks to email signature design tools, they’re easier than ever to create. Email header design isn’t an exact science—it’s decided by your brand’s style.

Analyze and take inspirations from the email design systems of top 22 brands. Color evokes emotional responses and sends signals to the brain. A yellow color calms the mind while a red alerts the reader. Understanding how different colors work in email is essential to convey your message.

We’ve got you covered with this professional email signature guide. This length ensures a full display on all types of screens. Andrian is a skilled web designer, email marketer, and SEO expert with over 20 years of experience.

Friday, May 3, 2024

Primer Designing Demonstration step by step

how to design primers for pcr

Primer-BLAST is run on a farm of machines at the NCBI to provide better service for users. Input can be a Genbank accession, a FASTA file, or even primers from another source. It also takes advantage of rich information from NCBI sequence databases to support other requirements such as placing primers based on intron/exon boundaries and SNP locations. Finally, it displays alignments between primers and targets found, allowing the user to make a decision on whether or not to use the primer pairs when potentially unintended targets exist. The Primer-BLAST program consists of a module for generating candidate primer pairs and a module for checking the target specificity of the generated primer pairs. Primer3 is used to generate the candidate primer pairs for a given template sequence.

Necessity for the design of a reference sequence

The forward primer creates copies of the 5’-3’ strand whereas the reverse primer makes copies of the complementary (runs 3’-5’) strand. To solve this type of problem in DNA replication or PCR experiment, primers are used, allowing the DNA polymerase to attach even in the middle of the lagging strand. The area replicated between two primers is called an Okazaki fragment. It is important that you do a quick consistency check -- you want to avoid having the added 5´ and 3´ flanking sequences base pair with the region of interest (ROI).

Authors’ original file for figure 5

In the SARS-CoV-2 RT-PCR assay, N1 and N2 were designed for the specific detection of SARS-CoV-2, whereas N3 was designed for the universal detection of SARS-like coronaviruses 51. Experiments found that the CDC N1 and N2 primer/probe sets performed better than the N3 set, and the N3 primer and probe set was removed from the Diagnostic Panel of revision 2 for the CDC 2019-nCoV Real-Time RT-PCR Diagnostic Panel. Note that this is not the concentration of oligos in the reaction mix but of those annealing to template. Primer3 uses this argument to calculate oligo melting temperatures. This parameter corresponds to 'c' in Rychlik, Spencer and Rhoads' equation (ii) (Nucleic Acids Research, vol 18, num 21) where a suitable value (for a lower initial concentration of template) is "empirically determined".

PCR Conditions

The validity evaluation of different 16srRNA gene primers for helicobacter detection urgently requesting to design new ... - Nature.com

The validity evaluation of different 16srRNA gene primers for helicobacter detection urgently requesting to design new ....

Posted: Fri, 24 Jun 2022 07:00:00 GMT [source]

For example, Primer-BLAST is the only tool that offers the ability to specify the number of mismatches that a specific primer pair must have to unintended targets and a custom 3’ end region where certain number of mismatches must be present. Furthermore, Primer-BLAST presents detailed alignments between the primers and targets found. We next compare the target detection sensitivity between Primer-BLAST and other primer design tools such as QuantPrime and PRIMEGENS. Ideally, a comparison of detection sensitivity would be to directly test the specificity checking modules across all tools using primers generated from a third party (for example, primers generated by Primer3). Unfortunately, this option is not available as Primer-BLAST is the only tool that offers specificity checking directly (i.e., specificity checking of pre-existing primers).

Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction

how to design primers for pcr

There are many other primer design tools available online, including primer3, and PCR suppliers often offer their own design programs free of charge. Zymo Research’s DNA Clean & Concentrator Kits were designed to facilitate the removal of salts, polymerases, and endonucleases from your PCR product, leaving you with purified, highly concentrated DNA. The resulting DNA is suitable for even the most sensitive downstream applications including sequencing, cloning, ligation, and endonuclease digestion. Discover which PCR purification kit best suits your research goals today. In RT-qPCR, RNA is first converted into cDNA (reverse transcription, or RT), and then amplified with PCR as described in the previous section. During maturation, RNA is subjected to splicing, which removes introns from pre-mRNA sequences.

The specificity checking module uses BLAST along with the Needleman-Wunsch (NW) global alignment algorithm [13] to look for matches between the primers and targets. The Primer-BLAST program is run on a farm of machines at the NCBI to provide faster and more reliable service to users. Several software programs, such as In-Silico PCR [8] and Reverse ePCR [9], do not design primers but rather determine the amplification targets of user-supplied primer pairs. However, even with the help of these tools, finding specific primers can still be a difficult process, because users often need to go through many candidate primers manually. The primer and probe sequences can also be designed using software tools 70,71. The commonly used primer design tools are listed in Table ​Table3.3.

HOW TO DESIGN PRIMERS FOR YOUR PCR EXPERIMENT

Primer design may start in silico, but it is ultimately proven at the bench. Here are the top recommendations for how to design primers for PCR and real time quantitative PCR (qPCR), reverse transcription quantitative PCR (RT-qPCR), bisulfite PCR, and methylation specific PCR (MSP). You can choose to exclude sequences in the selected database from specificity checking if you are not concerned about these. There are a large number of predicted Refseq transcripts in the Refseq mRNA, Refseq RNA and nr database. There are also many uncultured/environmental sample sequencesare in the nr database. This specifies the minimal number of bases that the primer must anneal to the template at 5' side (i.e., toward start of the primer) or 3' side (i.e., toward end of the primer) of the exon-exon junction.

The value of this parameter is less than the actual concentration of oligos in the reaction because it is the concentration of annealing oligos, which in turn depends on the amount of template (including PCR product) in a given cycle. This concentration increases a great deal during a PCR; fortunately PCR seems quite robust for a variety of oligo melting temperatures. The minimal number of contiguous nucleotide base matches between the query sequence and the target sequence that is needed for BLAST to detect the targets. Set a lower value if you need to find target sequences with more mismatches to your primers. Oligonucleotide primers are necessary when running a PCR reaction.

Sequence selected for designing forward primer (text highlighted in blue that is pointed by arrowhead). The red box indicates the parameters of the selected sequence like Tm, length of the sequence, and GC%. You can observe several parameters of the selected sequence from the header section of the tools (red box). The parameters are updated in live, so you can select or deselect the nucleotides to match the required criteria. The primer design steps remain the same even if you use a different tool. The following content focuses on demonstrating designing primers for different cloning methods.

how to design primers for pcr

You can run the complete sequences into programs like Vienna RNAfold   or NUPACK   as quick checks that the predicted structures of the RNAs remain the same with and without the flanking sequences. Understanding the reagents and what they are used for is critical in determining which reagents may be most effective in the acquisition of the desired PCR product. Adding reagents to the reaction is complicated by the fact that manipulation of one reagent may impact the usable concentration of another reagent. In addition to the reagents listed below, proprietary commercially available additives are available from many biotechnology companies.

The above tips are also applicable to a slightly different form of PCR called quantitative reverse transcription PCR (RT-qPCR). RT-qPCR allows you to perform PCR starting from RNA instead of DNA. If your RNA has been stored in TRIzol® or another similar reagent, learn more about Zymo Research’s Direct-zol RNA Purification Kits. These kits are made to efficiently purify total RNA that is suitable for RT-qPCR from TRI Reagent®, eliminating the need for chloroform, phase separation or precipitation steps. This specifies the max amplicon size for a PCR target to be detected by Primer-BLAST. In general, the non-specific targets become less of a concern if their sizes are very large since PCR is much less efficient for larger amplicons.

That is, as one parameter of PCR is changed, it may impact another. As an example, if the initial PCR was carried out at the sub-optimal annealing temperature (58°C) with an optimal Mg2+ concentration of 2.0 mM, then the result would produce a smear as seen in Figure 3c. An attempt to resolve the smear might involve setting up PCR conditions with reactions containing 2.0 mM MgCl2 and adjusting the annealing temperature to 61°C. Consequently, it is advisable to titrate reagents, rather than adding one concentration to a single reaction, when troubleshooting spurious results.

For cases where users submit a pre-existing primer pair to perform specificity checking (the primer-only case), an artificial template sequence is generated for the BLAST search by connecting both primers with a 20 base spacer region of N’s. This ensures that each primer will be treated separately in the BLAST search and thus achieves the equivalent effect of performing a separate BLAST search for each primer. To further minimize the search time, all regions on the template that do not contain candidate primers are masked out to avoid irrelevant BLAST hits. Since all candidate primer locations on the template are established by Primer3 already, amplification targets (amplicons) for all primer pairs can be identified using the single BLAST result above. A primer pair is deemed to be specific only if it has no amplicons on any targets other than the submitted template within the specificity checking threshold specified by the user.

(a) Self-annealing of primers resulting in formation of secondary hairpin loop structure. Note that primers do not always anneal at the extreme ends and may form smaller loop structures. (b) Primer annealing to each other, rather than the DNA template, creating primer dimers. Once the primers anneal to each other they will elongate to the primer ends.

For examaple, if you are only interested in targets that perfectly match the primers, you can set the value to 1. You can also lower the E value (see advanced parameters) in such case to speed up the search as the high default E value is not necessary for detecting targets with few mismatches to primers. To design a PCR primer set, a reference sequence is needed to identify the exact sequence being targeted and from where to select the primer pair candidates. Ideally, the reference sequence consists of not only the regions targeted for amplification, but also all the nucleotide sequences that will be involved in the amplification process.

Choosing appropriate primers is probably the single most important factor affecting the polymerase chain reaction (PCR). Specific amplification of the intended target requires that primers do not have matches to other targets in certain orientations and within certain distances that allow undesired amplification. The process of designing specific primers typically involves two stages. First, the primers flanking regions of interest are generated either manually or using software tools; then they are searched against an appropriate nucleotide sequence database using tools such as BLAST to examine the potential targets.

25 Spring Nail Designs to Screenshot for Your Next Manicure See Photos

flower design nail art

Pink Confetti Nails with Floral Accents celebrate your love for all things bright and sparkling. It’s like a party on your nails and, guess what, everyone’s invited — especially the flowers. If you’re tulip-obsessed, why not make those nails a springtime festival? Nails with Tulips can turn your fingertips into a Dutch wonderland.

Sweet Fields of Flowers

35 Clear Nail Designs You'll Want to Screenshot for Your Next Nail Appointment - InStyle

35 Clear Nail Designs You'll Want to Screenshot for Your Next Nail Appointment.

Posted: Sat, 21 Oct 2023 07:00:00 GMT [source]

But we’re here to make a case for softer colors this spring. This mix of pale and deep blues make for a really pretty base for your flower nail designs. Delicate pink, purple, and yellow shades create a playful palette for this almond-shaped floral manicure. The middle finger is embellished with gold hoops, and the index and pinky fingers each feature three beautiful flowers. These sweet and sassy stiletto nails boast painted flowers alongside pearly accents.

Groovy Floral Nail Art

It transitions well and is suitable for an important event or just a day to day look. Short, square nails are the strongest, longest-lasting shape. If you work in an office or find yourself typing often, this is your ideal shape. These ultra-glossy nails have boysenberry and magenta-colored flowers that look like a work of art all by themselves over a pearl blush base.

Minty Tips

In this design, the nude base is topped with a white lattice. Delicate magenta tulips with deep indigo stems and leaves dance across each nail, creating a miniature canvas of floral art that’s both quaint and captivating. Most of the nails in this design sport plain lavender polish.

Midnight Florals

Create accent nails that transition from soft pink to nude ombre, and add delicate pink flowers surrounded by white speckles on top to complete the look. To recreate this design, start with a nude base on all nails, then adorn three with a collection of green, pink, and blue flowers. Sport a manicure where delicate pink and white flowers bloom at the corners, accompanied by playful orange speckles, all against a sparkling nude backdrop.

Lucky You! St. Patrick's Day Nail Ideas You'll Love - Yahoo Life

Lucky You! St. Patrick's Day Nail Ideas You'll Love.

Posted: Thu, 25 Jan 2024 08:00:00 GMT [source]

O Christmas Nails, O Christmas Nails

Combine them with monochrome white nails and some dotted designs for an extra creative look. Can’t go wrong with a nail sticker or press-on option, especially if you’re on a time crunch (and implementing a strict budget). We love KISS’s Salon X-Tend Starter Kit, which gives you everything you need for your spring mani. If you’re looking for a softer, more whimsical approach to floral nail designs, you’ll love the delicacy of watercolor flower nails.

Yellow Negative Space Nails with Daisies

This mash-up of the classic French mani and mini orange and white flowers make for a bright spring mani that will put a smile on your face. So if you’re looking for some mani and pedi inspiration this spring (or beyond), below are 25 of the best flower nail art ideas. Never have we ever seen so many flowers painted onto nails—but we're here for it. Add drops of your chosen colors to each nail, using a dotting tool to drag them into five points.

flower design nail art

Then, paint a sheer, natural-colored base coat on the nails you want to add your flowers,” she says. “Add a top coat, and while it’s still wet, use tweezers to pick up and drop the dried flowers onto your nail. The gold leaf background really elevates this manicure and makes for a striking contrast to the delicate and minimal floral designs drawn at every corner. For detailed flower nail art in general, Goldstein likes to start by drawing the stem and leaves with a liner brush before mixing in your petal shades. Multiple colors, glitter, flowers, and crystals — this look has it all! The hot pink shade is paired with an alternating lighter, almost lavender-toned, pink on both hands.

Watercolor Flower Nails

Each nail is covered with branches of cherry blossoms mid-bloom. The pops of bubblegum pink in the blossoms contrast beautifully against the sky-colored background. It is intricate, feminine, and would look great on a wedding guest.

"Art representing trans rights and female reproductive rights are on trend and on message," Geer adds. Combine two ever-popular spring looks with slanted French tips and a retro flower design. Brought to life by Mabelyn Martin, the creative director of NYC nail salon Paintbox, it's part of the famed nail studio's Resort '23 capsule. You can request this look at the salon or re-create it at home using this convenient step-by-step tutorial. Get dramatic with these embellished flower designs, which feature chrome gold studs to add a little extra something to your everyday look.

Give your nails a shiny new lease on life by adding chrome floral accents to your pastel periwinkle French mani. The addition of the chrome offers a fresh take on a classic retro design. If you prefer the flowers that bloom at night, embrace a navy palette to capture that magically majestic feeling. Alternating solid nails with retro floral designs creates a sophisticated balance that's sure to boost your confidence. Claudia has been into dazzling nail designs ever since she was a kid, and now she’s fulfilling her dream of writing about them daily on TheCuddl. Claudia’s other passions include hiking, taking photos in nature, and trying out new recipes.

It’s the perfect way to enchant observers while you’re emphatically gesturing during a storytelling masterpiece. Glass Nails with Flowers bring that see-through sophistication with hints of blossoms. They’re the greenhouse you can flaunt when carting around an actual terrarium is frowned upon.

Thursday, May 2, 2024

Gilbert and Harvey Park Fandom

willow park owl house

Later, when the Abomaton returns from an errand Amity sent it on, Luz tries to stop it, only to accidentally set off an alarm it had in a panic. When Amity comes by and asks if she had touched it, Luz lies and says that she didn't, not wanting Amity to worry. Later, when Alador arrives and reveals that the alarm on the Abomaton had been set off, Luz starts acting nervous when Amity becomes worried upon finding out that she had lied to her and had been acting weird all day. Amity then runs off after getting upset at both her father and Luz, leading to Luz running after her.

Camila Noceda

In "Elsewhere and Elsewhen", Luz and Lilith use time pools to travel back to the Deadwardian Era in order to meet Philip Wittebane, who they don't know at the time would later become Belos, in order to get information on how to make a portal to the Human Realm. When Luz first sees Philip being harassed by two thugs, she immediately steps in to defend him. So, in order to get his attention, Luz offers to help him find the Collector. After arriving at a cave, Luz shows Philip the light glyph, and is surprised and excited to learn that he uses glyphs as well. Later, when they reach the door the Collector is behind, Luz decides to talk with Philip after Lilith starts to become skeptical of how uncomfortably familiar he seems.

List of The Owl House characters

When Luz goes to the library to deliver some of Eda's books and finds Amity reading to children, she tries again to fix things, but Amity refuses to do so. Luz there meets Amity's older siblings, Edric and Emira Blight, who also have a bad relationship with Amity, and they invite her to have fun, but Amity has them expelled from there. Edric and Emira tell Luz that they will come back at night for the Wailing Star that will happen that night, and they invite her to go with them, and Luz accepts when she sees that befriending them can be the way to befriend Amity. That night, they sneak into the library and discover that the Wailing Star gives life to the books, taking advantage of that to have fun.

Edric and Emira Blight

In "Escaping Expulsion", she started teaching her alongside Eda how to use glyph magic, and had no problems with bringing her with her to explore King's castle in "Echoes of the Past". In "Elsewhere and Elsewhen", Luz is shown to have become very fond of Lilith, even referring to her as a "cool aunt". While trying to find out more information about making a portal to the Human Realm, Luz asks Lilith for help in locating time pools so they can go to the Deadwardian Era in order to ask Philip Wittebane about the portal. In "Young Blood, Old Souls", Luz is enraged by what Lilith did to her own sister and goes on the warpath against her, even smashing a crystal ball showing a news broadcast she appears in.

Luz Noceda

Because of this, Luz sometimes has trouble taking him seriously, but when they both have to fend for themselves in "The Intruder", she gained a new appreciation for King when he opened up about feeling like a nobody, just like her. Luz is protective of King, being quick to call out Amity for insulting him during the events of "Covention". King also acts as a confidant of sorts to Luz as she will voice her concerns to him whenever she is too nervous to tell Eda something. Luz will occasionally ask for King's advice, despite it not always being that great. After Belos kills Luz, Eda's anger was so great that she gained a new Owl Beast form to avenge her.

When Belos leaves, Willow is on the verge of tears when Hunter is still out cold and is surprised to learn he is a grimwalker. She and the others watch as Flapjack sacrifices himself and Hunter wakes up. Willow and the others tell Luz they do not blame her for the Day of Unity and she, Gus, Hunter, Amity, Luz, and Camila go through the portal and into the Demon Realm. At the Halloween Festival, Willow, Hunter, Amity, Luz, and Gus walk around until they come to the Haunted Hayride. The five listen as Masha tells them about the legend of the Wittebane brothers as she and her friends listen with unease. After the ride she, Amity, and Gus lose track of Hunter and Luz as Amity realizes the rebus is missing.

They first met in "Separate Tides", when she temporarily joined his crew to take part in the hunt for the Selkidomus in order to improve Eda's struggling finances at the time. Bump is Luz's principal at Hexside whom she first met prior to her enrollment when she masqueraded as Willow's abomination project. Though Bump attempted to dissect her and later banned her from campus due to the chaos she inadvertently caused, Luz didn't hold any resentment towards him for this, and was deeply grateful when he permitted her to enroll as a transfer student. Since enrolling at Hexside, Luz has come to develop something of a love-hate student teacher relationship with Bump, similar to the one that her mentor Eda shared with him during her time as a student at Hexside. For her part, Luz respects Bump and generally considers him to be a good teacher, but can be frustrated at times by his strict adamance for following dogmatic rules and is unafraid of calling him out when she feels he is being unreasonable.

willow park owl house

She asks her if she had found any good ones, to which she replies that she hasn't, and Amity attempts to conjure up some with a plant glyph drawn in the ground, which doesn't work. Luz explains to her that she needs to have something in mind, and draws a proper one on the ground, summoning much prettier flowers. Amity then forms a balloon with abomination goo, and Luz ties the flowers around the string, letting them fly away with the balloon in the wind. Amity wonders if they'll reach the Human Realm, and while Luz is doubtful, she remarks that it's something nice to think about. In addition to her father's death, Luz struggled to adjust to life in Gravesfield throughout her childhood and into her teens, with most of the town's people resenting and ostracizing her for her antics and unusual behavior.

The Owl House: 5 Most Likable Characters (& 5 Fans Can't Stand) - Screen Rant

The Owl House: 5 Most Likable Characters (& 5 Fans Can't Stand).

Posted: Tue, 22 Sep 2020 07:00:00 GMT [source]

While scoping out students at the Club Fair, Hunter accidentally startles Puddles, who chases him through the air within sight of Willow's stand. She had been struggling to find enough players for her flyer derby team--witnessing Hunter outplaying Puddles with such strong flying skills impresses and excites Willow so much that she drags him toward her with plant magic, cheerfully introducing herself. At Flapjack's suggestion, Hunter introduces himself with Caleb's first name, not knowing its origins. Willow then hands him her team's pamphlet, which claims that the team will attract the best and the brightest witches--exactly the kind of recruits Hunter is looking for.

Over the course of the series, Willow has struggled with talking about her feelings, focusing on helping her friends with their problems and avoiding her own as a result. Willow wears a pair of round gold-framed glasses and the typical Hexside student uniform, consisting of a gray tunic and a dark gray cowl and belt with a gold pin, and gray-black short-heeled shoes. If you're going all in with your owl nesting box setup, look no further than this large box with an exercise platform.

In "Hooty's Moving Hassle", Luz is shown to be afraid of Eda's judgment and tries to stick to her rules so as not to be horribly punished. The following day, Luz helps Camila out at the veterinarian clinic and puts her egg under a heat lamp, hoping it would help it hatch. Shortly before they head out, Luz touches a glyph and it starts to burn slightly. When Luz and her mother arrive home, Hunter tells her he saw Belos and the pair search the old house to find only a possum. They conclude Belos is not there, but Luz promises Hunter she will keep him safe, saying that he is family now, and they head back to her house. The others are working on their Halloween costumes and Amity suggests she and Luz go as Hecate and Azura respectively.

After King and Eda explain that Hooty helped them, Luz says he was helping her ask Amity out. As King and Eda subdue Hooty, the girls fall into a small pit and the two ask each other out. They land in a forest and Luz finds the Golden Guard's staff along with an unconscious Golden Guard out, surprised that he is a teenager, and slaps him awake. They see Kikimora with the Golden Guard's mask, informing the group of scouts with her that he is dead, before secretly ordering the dragon that attacked earlier to find and end him. He and Luz slip away into the nearest town where the Golden Guard tries to order scouts to search the area for the missing palismen, However, they do not believe him and he demands Luz give him his staff.

Luz wakes up in a hallway lined with portraits as Hunter berates her for trapping them in the emperor's mind as they see a small child in a wooden mask. As Luz tries to convince Hunter of Belos' malicious actions, they see the shadowy figure approach them and Luz and Hunter hide in a memory. They see Belos preaching to a crowd about the coven system when "wild witches" attack. The memory continues, revealing the "attack" to have been explosives set off by a Golden Guard, with Belos ordering him to set off the rest. Next, they see Belos and another Golden Guard induct the first witches into the nine covens, and watch as they react negatively to the sigils and are left to die. They then see Belos speaking to the Collector, revealing their plan to kill the inhabitants of the Boiling Isles with a Draining Spell.

PDF Design of machinery : an introduction to the synthesis and analysis of mechanisms and machines

design of machinery

(610mm) wide, equipped on at least one side with a standard railing, shall be provided from the building exit door at the roof level to the means of access to the machine room or machinery spaces. Robert L. Norton's sixth edition of DESIGN OF MACHINERY continues the tradition of this best-selling book through its balanced coverage of analysis and design and outstanding use of realistic engineering examples. Topics are explained verbally and visually, often through the use of software, to enhance student understanding.

design of machinery

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Figure 4d illustrates the impact of each feature from the highest to the lowest. The analysis indicates that the feature α, which relates to the tortuosity and relative density by the Bruggeman equation, provides the highest impact on the electrical conductivity, followed by the specific surface area SA, and β. The results provide guidelines for the microstructure design, uncovering the most critical microstructural features for the electrical conductivity. The MVLR model provides information about the interplay but is not suitable to predict the microstructure with high accuracy. It may rather provide an estimate about the spatial extent in context to the microstructural feature space. For the definite reconstruction of the microstructure, we utilize a conditional GAN (cGAN) model and diffusion-based model (DDPM).

Learning Solutions

SAHPB and SANPC decays in a more constant manner with temperature. Design of Machinery has proven to be a favorite of both students and educators across the globe. It is currently used in hundreds of schools in the U.S. and Canada and in many more worldwide in both English and several other languages.

Structure Synthesis of Spatial Mechanisms

Such generated reconstructed synthetic images can be further utilized for morphological analysis16,60,61. A promising approach to reconstruct the microstructure for a given material parameter is by utilizing deep generative models14,54,55. In particular, we apply a denoising diffusion probabilistic model (DDPM) architecture.

Image pre-processing

This height may be reduced to 3 ft (914mm) between the machine beams and the sheave space floor. (A) Where the floor of the machine room or of the machinery space is more than 8 in. (203mm) above or below the floor or roof from which the means of access leads, stairs, or ladders shall be provided between such levels. Permanent means for safe and convenient access shall be provided to all machine rooms, overhead sheave spaces provided with a floor, and secondary levels. But the real reason we want to bring stiffness into (especially) milling machines is due to resonant chatter that often arise inside of the 1-15kHz frequencies.

§3011. Machine Rooms and Machinery Spaces.

Yet, a major difficulty for a proper assessment of the microstructure-property relationship often lies in the accuracy and statistical pertinence of the extracted microstructure features. Indeed, the developed unique workflow paves the way towards machine learning driven accelerated material design. The findings in this paper are not only limited to the conductivity prediction of sintered porous materials but also suggest broader applications to other porous microstructures and material properties. In particular we validate the model performance by comparing the evaluated relative density, specific perimeter, as well as shape index for the real and synthetic microstructures in relationship to the sinter temperature, see Fig.

Virtual design may facilitate powertrain electrification for agricultural machinery University of Helsinki - Universitas Helsingiensis

Virtual design may facilitate powertrain electrification for agricultural machinery University of Helsinki.

Posted: Fri, 08 Feb 2019 08:00:00 GMT [source]

However, the trouble with laying elements right on top of eachother so as to be perfecty aligned is that they interfere. We cannot put the X axis directly through the workpiece, for example. So we will typically find that machines with large build volumes have larger “misalignments” between the work being done and the drives doing the work, i.e. in the shopbot above. In machines with typically small build volumes (as a fraction of total machine size) we will find that load-to-drive-offsets are small (again relative total machine size).

Tortuosity measurements

By supporting multiple S3 buckets as data sources, the need for creating multiple knowledge bases or redundant data copies is eliminated, thereby optimizing cost and promoting cloud financial management. Furthermore, the cross-account access capabilities enable the development of resilient architectures, aligning with the Reliability pillar of the AWS Well-Architected Framework, providing high availability and fault tolerance. Based on the segmented image data utilizing the U-NET with the hybrid training we perform the curvature analysis. The shape index used for the analysis of the real and synthetically reconstructed image relates to the local curvature measurement. Its value lies between -1 and 1, where 1 represents ‘spherical caps’72.

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Consequently, machine learning (ML) has been used to identify the influence of chemical structures ranging from sub-angstrom-level to gross-level in relation to the property of interest1. Deep learning has been used to predict material properties, e.g., ionic conductivity7, or mechanical properties8,9. A Segmented volume of interests (VOIs) with 10 × 10 × 10 μm3 for sample HPA, HPB and NPC, exemplary for 175 °C, with the copper (gray) and pore (red) phases. B Evaluated relative density D as a function of the sinter temperature for HPA (blue), HPB (gold) and NPC (red) extracted from the segmented VOIs. C Electrical conductivity σ vs. relative density D for HPA (blue), HPB (gold) and NPC (red). D Skeletonized copper phases illustrate the 3D copper struts distributions for sample HPA, HPB and NPC between 175 °C and 400 °C.

The next step is to assess the quality of the reconstructed synthetic images. As illustrated here16, Frechet inception distance (FID) as well as precision and recall are not suitable to measure the quality of synthetic microstructure data. Here, we assesses the quality of the synthetic images based on extracted physical descriptors of the microstructure or microstructural features57. A, d and g illustrate the segmented microstructures for the porous materials HPA, HPB, and NPC obtained with FIB-SEM. B, e and h correspond to the predicted (synthetic) microstructures utilizing the cGAN model for HPA, HPB, and NPC, respectively. The data visualized in c, f and i correspond to the predicted (synthetic) microstructures utilizing the DDPM for HPA, HPB, and NPC, respectively.

The synthetic and experimentally retrieved (real) microstructures are plotted for different sinter temperatures. The segmented copper and pore phases are illustrated in white and black, respectively. The frame colors are related to the porous materials (HPA, HPB and NPC), real and predicted microstructures. A Prediction results for MVLR model A, C and I versus the measured electrical conductivity. Here a linearity is provided from 10 to 200 uS.cm−1, which is not for the whole experimental window. We validate the models’ performance with three test sets, indicated by Test A, C and I, not used for the training, to find the best model.

Depending on your needs, you can choose either compact or heavy-duty air compressor frames. SOLIDWORKS welcomes your feedback concerning the presentation, accuracy, and thoroughness of the documentation. Use the form below to send your comments and suggestions about this topic directly to our documentation team. The documentation team cannot answer technical support questions.

Professor Norton responds by providing a CD with Working Model and by my count about 150 example files, TKSolver with about 30 examples, and some 100 examples of his own programs and handouts. This patchwork of software is a valiant attempt to fill a critical need for software tools for machine theory education. Most engineers use a computer for analysis, drawing, and report generation, so why are computer algebra, drawing and animation not more a part of our machine theory curriculum?

As a result, the emerging tails in QI are getting less pronounced by increasing the temperature for all presented materials. The observed behavior is complemented with an increased number of cup-concave geometries indicated by more pronounced tails in QII. For the evaluation of the copper strut diameter ϕ and its evolution upon sintering, we skeletonize and statistically analyze the segmented data24 (Fig. 2d, e). The mean values are obtained by fitting the log-normal distribution of the strut diameter histograms (Supplementary Note 4). This behavior is linked to the continuous growth of the bonds between the sinter particles due to the increase in temperature45.

Luz Noceda Fandom

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From an early age, Luz's eccentricities resulted in her being unable to make any friends, as other children found her weird and repulsive, while also receiving disgust from adult onlookers who would regularly openly criticize her for her behavior. In school, her antics were often disruptive, regularly getting her in trouble with the faculty and landing her in the principal's office on several occasions. During this time, Luz was also bullied by her classmates, who frequently made fun of her for her antics and "cheesy" stunts to impress other students that she developed crushes for.

Building a portal and dating Amity

Later, both Luz and Amity, along with Willow, Gus, Hunter, sneak into the Blight factory, and get captured by Odalia and Kikimora instead. In "Knock, Knock, Knockin' on Hooty's Door", it's shown that Luz is torn between trying to get information out of the Echo Mouse and trying to figure out how to ask Amity out, referring to her as a "cotton-candy-haired goddess". Luz is worried that Amity will find her cheesy like everyone did back home, stating that Amity is "smart, cool, and classy", so asking her out should be breathtaking, emotional and real. Later, she finds Amity in the basement, who has been kidnapped and brought there by Hooty. When Amity mentions wanting to forget about the kiss at Blight Manor, Luz gets worried and quickly changes the subject.

Season One

On her part, Luz generally considers Kikimora to be a legitimate yet thoroughly unimpressive villain, often treating her as more of a nuisance compared to much more powerful adversaries like Belos and the Collector. Luz threatening to immolate Kikimora unless she releases her imprisoned friends. When the Collector becomes frustrated about losing every game and goes to sulk in a corner, noticing their sadness, Luz goes to talk to him, managing to get the child to open up about his insecurities and anger of being used and abandoned by everyone they knew. Understanding now how the Collector feels and how he never meant any genuine harm to anybody, Luz takes him on a trip around the Boiling Isles, demonstrating how she bonded with Eda and King and teaching them how kindness and forgiveness goes a long way.

Edric and Emira Blight

These experiences resulted in Luz becoming fearful of interacting with other teenagers. When they later reach a memory of Belos talking with the Collector, Luz is shocked to find out that he has been rebuilding the portal door, as well as learning that he plans to use the Draining Spell on the Day of Unity in order to wipe off the inhabitants of the Isles. Later, she and Hunter are shocked when they find out that "Inner Belos" was actually the palisman souls he had consumed, and realize that the real Inner Belos was the child version that had been with them. After Luz threatens to tell everyone the truth about the Day of Unity, she is shocked to learn that Belos is actually a witch hunter.

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During this time, Luz and Vee were able to bond as friends, with them and the others often playing together and enjoying each other's company over the summer. In addition to their growing friendship, it's implied that, after learning more about Luz's reasons for running away and the circumstances that made life on Earth so hard for her, Vee harbors guilt over her previous condemnation of her, and seeks to make it up to her. This was demonstrated by how, prior to her return, Vee returned all of Luz's belongings that she had earlier attempted to throw away to their proper place in addition to leaving a note on her bedroom door, welcoming Luz home. For her part, Luz has been shown to bear no ill-will towards Vee for her previous theft of her identity, nor her earlier criticism, instead welcoming her as part of her family and as one of her friends. She admires and looks up to Eda, hoping to be a great witch like her one day.

Amity noticed Luz's frowned expression, prompting her to cast a light spell for her. Once Willow's memory is restored in the former episode, Luz welcomes her back with an eager hug, stating that "[she'd] hug her so hard, she'd never forget [Luz] again". In "King's Tide", King saves Luz and her friends from possibly being killed by the Collector after they are released, saying that they're needed alive for the game of "Owl House". Luz, seeing what King is doing, plays along, leading to the Collector stopping the Draining Spell. As the Collector starts reshaping the island, Luz tries to keep the portal together long enough for the others to escape.

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Inner Willow then pulls Luz and Amity into the memory where it shows Amity pushing Willow away and Inner Willow tries to destroy Amity, until Amity reveals how her parents had forced her to end the friendship. Luz watches as Amity apologizes and promises to be a better person in the future. After exiting Willow's mind, Willow questions Luz on how Amity found out about the memory in the first place. When Luz and Willow are in photo class, they extract memories from each other and Luz becomes curious upon seeing a memory of Willow and Amity, to which Willow dismisses it as the past and asks Luz not to ask about it more before reversing the photo.

That day at school, the head of the Illusion Coven, Adrian Graye Vernworth, comes to Hexside, informing the students that Belos wants all young witches branded before the Day of Unity, also claiming that he will give them fake ones for protection. However, Gus sees through the ruse and reveals that Adrian is tricking them into being branded. When the Coven Head, who has brought Coven Scouts with him, is about to give Gus an abomination sigil, the boy creates a labyrinth-like illusion across the school that allows the students to escape. As Willow and Amity run through the halls, Amity learns everyone is in the healing homeroom, and she and Willow try to get there.

Episode information

As they get ready to head out, Luz and King hug Eda goodbye and she makes Raine promise to watch out for Eda. When the carriage stops, Luz jumps out ready for a fight, only to be restrained by Darius Deamonne and freed by Eberwolf. She looks around and sees Eda, King, Lilith, Hooty, Steve, Katya, Derwin, and Amber. Eda explains that everyone present was suspicious of the Day of Unity and have formed a group, the Covens Against the Throne, to stop it. After going to their headquarters in Latissa, Eda presents Luz with a box labeled "Eda's longest toenails" and, upon evening, is surprised to see the palistrom wood in it.

However, this ends with Eda's palisman, Owlbert, getting a crack down his head. He flees into the woods and the three of them go looking for him, despite Willow's protests. They soon confront the Bat Queen and her babies, who trap Willow and Gus in webbing, and subject Luz to doing trials in order to win back Owlbert's trust. After the trials, the Bat Queen traps Owlbert and begins to battle with Luz, while Willow and Gus deal with the Bat Queen's babies. After the truth of the Bat Queen being a palisman is revealed, she flies off, leaving the trio to celebrate.

She watches as Lilith takes on half of Eda's curse and, when the sisters reveal they have lost their magic, Luz offers to teach them about glyphs. As King reads Luz The Unauthorized Boiling Isles History, she decides to save Eda despite King trying to talk her out of it. After watching a news report that Eda will be petrified, an irreversible process. Luz says it should be Lilith as King explains the severity of one's crimes in order to get petrified. The two get sent to the Conformatorium where they take on the guards and find where Eda is being kept. Luz uses alight glyph to snap Eda back to her senses, and Eda tells Luz to take the key to the Human Realm in order to keep it away from Belos.

Amity used to be Willow's friend until she stopped hanging out with her when she got her witch powers and Willow didn't. Due to their strained relationship, Amity often taunts her, often leaving her seething in anger. She wants to prove Amity wrong, and that she is a strong witch in her own right. In "Understanding Willow", all of this changes when Amity is confronted by Willow's conscience, her "inner Willow" in her mind. After feeling all the guilt, Amity reveals why she stopped being friends with Willow.

They toss a blue log in her lap and reveal they raided the Bonesborough Garden Club for palistrom wood so she can carve her own palisman. When Willow gets hurt after Gus fails to lure away pixies, Luz comforts him. The following day, needing a library card to get into the Bonesborough Library, Luz goes to Gus, who knows what she is after. As they walk to the library, Gus laments about his actions with the pixies and thinks about changing tracks when they see a group of Glandus High students defend a child from a slitherbeast. When they say they are looking for the Looking Glass Ruins, Luz urges Gus to go with them and gives him some glyphs.

Wednesday, May 1, 2024

L A. council member to seek more money to help Chinatown tenants Los Angeles Times

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Seventeen of the building’s units are occupied by residents who are paying market rate. An additional 68 units already have been subsidized by Section 8 rental vouchers — although some of those tenants have also withheld rent, Botz said. The agreement approved Friday would roll back rent hikes in many of Hillside Villa’s units.

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Sewill, whose home was picketed multiple times, eventually came out against that idea, saying the cost of acquiring the property from an unwilling seller, then fixing and refinancing it, would reach nearly $93 million, or $748,665 per unit. Thomas Botz, managing member of 636 NHP, the company that owns the building, said the unpaid rent ranges from less than $5,000 for some households to nearly $91,000. After Friday’s vote, Botz praised the council member for working to find a resolution.

A housing shortage in the U.S. is leading to zoning changes - NPR

A housing shortage in the U.S. is leading to zoning changes.

Posted: Sat, 17 Feb 2024 08:00:00 GMT [source]

Opinion: Renting in L.A. could go from bad to worse

A studio apartment is currently $2,199, and we use a budgeting rule that says rent should not be more than 30% of your total income. So $70,000 would not be enough to live in LA at the average rate; you would want to earn closer to $90,000 a year. Finding a pet-friendly apartment in Los Angeles can feel overwhelming, but don't despair!

College protesters want ‘amnesty.’ At stake: Tuition, legal charges, grades and graduation

While the city boasts a vibrant mix of cultures and energy, pet policies can vary significantly. On average, expect to pay around $2,199 per month for a studio apartment in Los Angeles. All three families are considering wrongful death lawsuits, Fox News Digital previously reported. The source also said Willis has not returned to work as a data scientist at HIV research group IAVI. A paranoid Lawrence accused management, maintenance workers and others living in the complex of entering his home, turning lights on and off and rearranging furniture. Hernandez said she is looking for other funding sources to cover the remaining back rent.

city house apartments

Councilmember Eunisses Hernandez, whose district includes Chinatown, said this week that she had persuaded the owner to delay the start of the six-year repayment period by six months. She said she also intends to use $250,000 of her office’s discretionary funds to erase the first year of unpaid rent debt at Hillside Villa, a move that could delay the start of the repayment process until late 2025. The agreement, if approved, would keep many rents affordable inside Hillside Villa, but also scuttle efforts to have the city acquire the building. For years, a major portion of the building’s tenants have participated in a rent strike, withholding payments in an effort to counter steep rent increases. According to our rental estimate data, as of March 2024, rent prices are going down in LA. They were down by 3.4% year over year even though they had risen slightly over the previous month by 0.5%.

L.A. cuts tentative deal to provide $15 million to spare Chinatown tenants from steep rent hikes

Moving to Los Angeles is the continuation of the American dream, of traveling west towards the setting sun and carving out a life on the edge of the world. Renting an apartment in LA is actually easier and less expensive than in cities of comparable size like San Francisco and New York. Legions of dreamers, wanderers, pioneers, artists and those in search for a better life have picked up and moved to the belly of the beast, and so can you. "I was talking to a manager, he was getting me some paint for my room, and the guy just came up to her, who lived there, and shot her four times," said Joe Guerrero.

Unless otherwise specified in writing, Broker/Agent has not and will not verify any information obtained from other sources. The Broker/Agent providing the information contained herein may or may not have been the Listing and/or Selling Agent.

Rental Resources

The average rent for a 1-bedroom apartment in Los Angeles is approximately $2,848 per month. Witnesses say a resident, who's been identified by police as 67-year-old Brian Lawrence, walked up to a manager and shot her several times. As part of the deal, Botz will have an additional 10 years to repay a $3.5-million redevelopment loan at 1% interest. A second, $1.85-million loan will eventually be forgiven, as long as the landlord adheres to the terms of his agreement with the city, housing officials said.

L.A. council member to seek more money to help tenants in Chinatown apartment building

Tenant activists responded by urging the council to purchase Hillside Villa using eminent domain, a process used by government agencies to acquire private property. Now that you’ve been outfitted with the proper tools, tip, and tricks, we’re confident that finding the LA apartment of your dreams is definitely within reach. LA is a big place gleaming with opportunity, and it’s up to you to grab it, like life, by the horns. Now go forth, dear apartment hunter, and claim your piece of this angelic city.

Hillside Villa had served for 30 years as affordable housing as part of an agreement with city officials. Those rent restrictions expired five years ago, prompting Botz to increase rents, in some cases doubling or tripling the amount. Rent prices listed are averages and may not reflect the cost of specific units. Rental prices can vary depending on factors like size, location, amenities, and availability. Fox 4 also reported that the attorney of Alex Lee, a fifth man who spent time at Willis’ house with the men in their final hours, had been contacted by the prosecutor’s office in the case.

Willis moved out of his home on Northwest 83rd Terrace, moved his belongings into storage and checked into a rehab facility shortly after his friends’ deaths, a source close to him previously told Fox News Digital. Police along with other city partners have reached out to residents and staff at the complex to provide counseling and other resources. An hours-long search in the apartment complex that night when SWAT officers entered Lawrence's apartment and found him dead from a self-inflicted gunshot wound, police said.

In addition, tenants with unpaid back rent would avoid eviction proceedings by signing an agreement promising to gradually repay the landlord over the next six years at 3% interest, according to the city’s report. The Los Angeles City Council voted Friday to spend nearly $15 million to spare many tenants of a Chinatown apartment building from major rent hikes, even as several of those residents demanded the city do more to erase their unpaid back rent. Furthermore, major rental listing websites like Apartment List allow filtering by pet-friendliness. Simply select “Dog-Friendly” or “Cat-Friendly” in your preferences when you start your search. ANAHEIM, Calif. (KABC) -- A dispute may have led to an apparent murder-suicide in which a tenant shot and killed a manager at an Anaheim apartment complex, and residents in the surrounding community are in shock.

Housing officials reported last week that they had struck a deal with Botz to use city money to roll back rent for many Hillside Villa tenants to 2019 rates. Tenant advocates and some residents of Hillside Villa denounced the deal, saying many cannot afford to pay. Residents in 48 of the building’s 124 units still owe a combined $1.4 million in back rent, according to Ann Sewill, who heads the city’s Housing Department. A 3-bedroom apartment in Los Angeles will cost, on average, $4,581 per month. In Los Angeles, a 2-bedroom apartment typically rents for around $2,962 per month.

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